王 庆，女，研究员/主任，博士生导师，农业农村部草鱼出血病首席专家，全国水产标准化技术委员会水生动物防疫工作组委员，广东省现代农业产业技术体系创新团队疫病防控岗位专家。主要从事水产动物病害防控技术研究，以草鱼出血病、锦鲤疱疹病毒病等淡水鱼重要病害为研究对象，系统开展了流行病学、诊断技术开发、疫苗创制等方面的研究。主持国家自然科学基金，广东省科技计划重点项目，动物疫情监测与防治(渔业）项目，国家科技支撑计划子课题，水产行业标准，中国水产科学研究院基本科研业务费专项资金项目等20余项。参与国家科技支撑项目、国家自然科学基金，现代农业产业技术体系，农业部948计划项目，广东省科技计划等项目十余项，经费超过3000万元。获得省科技进步二等奖1项、水科院科技进步三等奖1项。发表论文90余篇，其中SCI 39篇，授权国家发明专利15项，制定国家标准、行业标准及地方标准4项。参与编写专著6本，指导培养研究生12名。目前为上海海洋大学博士生导师，天津农学院、华南农业大学、西南大学学术型硕士生导师。 教育、进修经历 1995.09-1999.06 华南农业大学 兽医学院 兽医专业 学士 1999.07-2002.06 华南农业大学 兽医学院 预防兽医学专业 硕士 2005.09-2008.06 中山大学 生命科学学院 水生生物学专业 博士 2013.08-2013.09 德国动物健康研究院病原学研究所 学术交流 2014.11-2015.02 德国动物健康研究院病原学研究所 技术培训 2015.03-2016.03 美国俄勒冈州立大学 访问学者 工作经历 2002.07-2005.08 广东睿诚达贸易有限公司，中级兽医师 2008.07-至今 珠江水产研究所，从事水产动物病害防控研究，科研 代表性科研项目 1. 国家自然科学基金项目：基因II型草鱼呼肠孤病毒预测表面纤突与连接粘附分子GcJAM-A的相互作用研究（31602202，2017-2019年），19万，主持； 2. 广东省科技计划重点项目：草鱼出血病二价灭活疫苗的研制与应用示范（2016B090918115，2016-2018年），100万，主持； 3. 广东省省级促进经济发展专项资金（海洋经济发展用途）项目：基于芽孢杆菌呈递系统的鱼类新型疫苗研究及应用（2019-2021年），100万，主持； 4. 广东省现代农业产业技术体系创新团队建设专项资金，病害防控岗位（2019-2021），5.6万/年，主持； 5. 农业部财政项目：动物疫情监测与防治(渔业）项目－草鱼出血病风险评估（2015-2020），280万，主持； 6. 水产行业标准项目: 鱼类细胞系第14部分：锦鲤吻端细胞系(KS)（2016年），6万，主持。 代表性论文、著作 1. Hua Gong, Qing Wang*, Yingtiao Lai, Changchen Zhao, Chenwen Sun, Zonghui Chen, Jiafa Tao*, Zhibin Huang. Study on Immune Response of Organs of Epinephelus coioides and Carassius auratus After Immersion Vaccination with Inactivated Vibrio harveyi Vaccine. Frontiers in Immunology, 2021, 11:622387. 2. Jiaming Chen, Yingying Li, Yingying Wang, Siyu Wu, Ouqin Chang, Jiyuan Yin, Weiwei Zeng, Sven M. Bergmann, Qing Wang* Establishment of a rare minnow (Gobiocypris rarus) model for evaluation of experimental vaccines against a disease induced by grass carp reovirus genotype II. Fish and Shellfish Immunology, 2021, 117:53-61. 3. Caixia Gao, Yingying Wang, Huzi Hu, Wenli Zhou, Jiyuan Yin, Yingying Li, Sven M. Bergmann, Siyu Wu, Weiwei Zeng, Qing Wang* Assessment of a natural grass carp reovirus genotype II avirulent strain GD1108 shows great potential as an avirulent live vaccine. Microbial Pathogenesis, 2021,152:104602. 4. Jiaming Chen, Ouqin Chang, Yingying Li, Yingying Wang, Chao Liu, Jiyuan Yin, Sven M. Bergmann, Weiwei Zeng, Qing Wang*. Establishment of a rare minnow (Gobiocypris rarus) disease model for grass carp reovirus genotype II. Aquaculture, 2021,533:736133 5. Ting Gao, Caixia Gao, Siyu Wu, Yingying Wang, Jiyuan Yin, Yingying Li, Weiwei Zeng, Sven M. Bergmann and Qing Wang*. Recombinant Baculovirus-Produced Grass Carp Reovirus Virus-Like Particles as Vaccine Candidate that Provides Protective Immunity against GCRV Genotype II Infection in Grass Carp. Vaccines, 2021, 9:53 6. Yuru Yang, Yingying Wang*, Qing Wang**, Weiwei Zeng, Yingying Li, Jiyuan Yin, Siyu Wu, Cunbin Shi. Establishment of a cell linefrom swim bladder of the Grass carp (Ctenopharyngodon idellus) for propagation of Grass carp reovirus genotype II. Microbial Pathogenesis, 2021,151,104739. 7. Zhishen Huang, Yingying Wang, Siyu Wu, Jiyuan Yin, Wenli Zhou, Ting Gao, Yingying Li, Sven M. Bergmann, Caixia Gao, Yahui Wang, Weiwei Zeng, Qing Wang*. An iTRAQ-based comparative proteomic analysis of grass carp infected with virulent and avirulent isolates of grass carp reovirus genotype II. Aquaculture, 2021, 535: 736426. 8. Yingying Li, Qing Wang*, Feng Hu, Yingying Wang, Sven M. Bergmann, Weiwei Zeng, Jiyuan Yin,Cunbin Shi. Development of a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for the detection of KHV. Journal of Fish Diseases, 2021,44(7):913-921. 9. Feng Hu, Li Yingying, Wang Qing*, Zhu Bin, Wu Siyu, Wang Yingying, Zeng Weiwei, Yin Jiyuan, Liu Chun, Bergmann Sven M., Shi Cunbin. Immersion Immunization of koi (Cyprinus carpio) against Cyprinid herpesvirus 3 (CyHV-3) with carbon nanotube-loaded DNA vaccine. Aquaculture, 2021,539:736644 10. Jiaming Chen, Yingying Li, Yingying Wang, Siyu Wu, Ouqin Chang, Jiyuan Yin, Weiwei Zeng, Sven M. Bergmann, Qing Wang*. Establishment of a rare minnow (Gobiocypris rarus) model for evaluation of experimental vaccines against a disease induced by grass carp reovirus genotype II. Fish and Shellfish Immunology, 2021, 117:53-61 11. Yingying Wang, Qing Wang, Weiwei Zeng*, Yingying Li, Jiyuan Yin, Sven M. Bergmann, Xinping Zhu*. Establishment of a brain cell line obtained from hybrids of Channa argus ×Channa maculata for the detection of tilapia lake virus. Microbial Pathogenesis, 2020, 138:103810. 12. Feng Hu, Yingying Li, Qing Wang*, Gaoxue Wang, Bin Zhu, Yingying Wang, Weiwei Zeng, Jiyuan Yin, Chun Liu, Sven M. Bergmann, Cunbin Shi. Carbon nanotube-based DNA vaccine against koi herpesvirus given by intramuscular injection. Fish and Shellfish Immunology, 2020,98: 810-818. 13. Yingying Wang, Qing Wang*, Yingying Li, Jiyuan Yin, Yan Ren, Cunbin Shi, Sven M. Bergmannc, Xinping Zhu, Weiwei Zeng. Integrated analysis of mRNA-miRNA expression in Tilapia infected with Tilapia Lake virus (TiLV) and identifies primarily immuneresponse genes. Fish and Shellfish Immunology, 2020, 99: 208–226. 14. Ye Ge, Qiucheng Yao, Yingying Wang, Qing Wang*, Weiwei Zeng. Deep illumine miRNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection. Aquaculture Research, 2020, 52(2): 463-470. 15. Yingying Li, Qing Wang*, Sven M. Bergmann, Weiwei Zeng, Yingying Wang, Yan Ren, Cunbin Shi, Dangen Gu. Preparation of monoclonal antibodies against KHV and establishment of an antigen sandwich ELISA for KHV detection. Microbial Pathogenesis, 2019, 128:36–40. 16. Shixu Liu, Qing Wang*, Yingying Wang, Jiaming Chen, Ouqin Chang, Weiwei Zeng, Sven M. Bergmann, Yingying Li, Jiyuan Yin, Hong Wen. Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II. Microbial Pathogenesis, 2019, 136:103715. 17. Weiwei Zeng, Yingying Wang, Yanmin Guo, Sven M. Bergmann, Jiyuan Yin, Yingying Li, Yan Ren, Cunbin Shi, Qing Wang*. Development of a VP 38 recombinant protein‐based indirect ELISA for detection of antibodies against grass carp reovirus genotype II. Journal of Fish Diseases, 2018, 1811-1819. 18. Qing Wang, Hualiang Xie, Weiwei Zeng*, Linchuan Wang, Chun Liu, Jiexing Wu,Yingying Wang, Yingying Li, Sven M. Bergmann. Development of indirect immunofluorescence assay for TCID50 measurement of grass carp reovirus genotype II without cytopathic effect onto cells. Microbial Pathogenesis, 2018, 114: 68–74 19. Weiwei Zeng, Wei Yao, Yingying Wang, Yingying Li, Sven M. Bermann, Yan Ren,Cunbin Shi, Xinjian Song, Qiwen Huang, Shuchen Zheng, Qing Wang∗.Molecular detection of genotype II grass carp reovirus based onnucleic acid sequence-based amplification combined withenzyme-linked immunosorbent assay (NASBA-ELISA). Journal of Virological Methods, 2017,243 :92–97 20. Shucheng Zheng, Qing Wang*, Sven M. Bergmann, Yingying Li, Weiwei Zeng, Yingying Wang, Chun Liu, Cunbin Shi. Investigation of latent infections caused by Cyprinid herpesvirus 3 in koi (Cyprinus carpio) in southern China. Journal of Veterinary Diagnostic Investigation, 2017, 29(3): 366–369. 21. Shucheng Zheng, Yingying Li, Qing Wang*, Jiexing Wu, Yingying Wang, Weiwei Zeng, Sven M. Bergmann, Yan Ren, Cunbin Shi. Identification of one novel envelope protein encoded by ORF136 from Cyprinid herpesvirus 3. Archives of Virology, 2017, 162: 3849–3853. 22. Yingying Wang, Weiwei Zeng, Qing Wang*, Sven M. Bergmann, Yingying Li, Shucheng Zheng, Yan Ren, Chun Liu, Ouqin Chang, Peiyu Lee. Establishment and Characterization of a new Cell Line from Koi Brain (Cyprinus carpio L.). Journal of fish disease,2017, 41(2):357-364 23. Yingying Li, Shucheng Zheng, Qing Wang*, Sven M. Bergmann, Weiwei Zeng, Yingying Wang, Chun Liu, Cunbin Shi. Detection of Cyprinid herpesvirus 3 using a monoclonal antibody against Cyprinus carpio IgM. Archives of Virology, 2017:1-5. 24. Qing Wang, Weiwei Zeng*, Liang Yin, Yingying Wang, Chun Liu, Yingying Li, Shucheng Zheng, Cunbin Shi. Comparative study on physical-chemical and biological characteristics of grass carp reovirus from different genotypes. Journal of The World Aquaculture Society, 2016, 47(6): 862-872. 25. Yingying Wang, Weiwei Zeng, Yingying Li, Hongru Liang, Chun Liu, Houjun Pan, Peiyu Lee, Shuqin Wu, Sven M. Bergmann, Qing Wang*. Development and characterization of a cell line from the snout of koi (Cyprinus carpio L.) for detection of koi herpesvirus. Aquaculture, 2015, 435: 310–317. 主要获批专利 1. 王庆，王英英，李莹莹，尹纪元，石存斌，任燕，吴斯宇。KIT AND METHOD FOR DETECTING VIRUS TITERS IN GRASS CARP REOVIRUS GENOTYPE II VACCINE。专利号：2020103866，授权时间：2021.1.27（澳大利亚国际专利） 2. 王英英，王庆，曾伟伟，李莹莹，尹纪元，吴斯宇，石存斌。草鱼呼肠孤病毒II型巢式RT-PCR检测引物组、试剂盒及其应用。专利号 ZL 2020109152965，授权时间：2021.7.20（国家发明专利） 3. 曾伟伟，王庆，王英英，尹纪元，汤亚方，石存斌，张德锋，李莹莹，任燕，常藕琴。一种罗湖病毒的RT-LAMP检测引物组、试剂盒及方法。专利号：ZL 201711282120.5， 授权时间：2021.7.20（国家发明专利） 4. 王英英，王庆，李莹莹，尹纪元，吴斯宇，石存斌，任燕。Gobiocypris Rarus Swim Bladder Cell Line And Culture Method And Induced Transformation Method Thereof. 专利号： 2020102488. 授权时间：2020.12.20（澳大利亚国际专利） 5. 曾伟伟，王庆，王英英，李莹莹，石存斌，任燕，黄志斌。一种草鱼呼肠孤病毒II型疫苗株和野毒株鉴别引物及含有其的试剂盒与诊断检测方法，专利号ZL 201710143487.2。授权时间：2020.09.18（国家发明专利） 6. 尹纪元，王庆，曾伟伟，王英英，李莹莹，黄聪，渠洋。具有特定病原微生物拮抗能力的草鱼源乳酸菌及其应用。专利号：ZL 2019 10977632.6，授权时间：2020.7.21（国家发明专利） 7. 王英英，王庆，曾伟伟，李莹莹，尹纪元，任燕，常藕琴，石存斌。一株乌斑鳢脑细胞系的建立及应用。专利号：ZL 201910743467.8，授权时间：2020.6.15（国家发明专利） 8. 王英英，王庆，曾伟伟，李莹莹，尹纪元，常藕琴，石存斌。一株乌斑鳢肾细胞系及其构建方法和应用，专利号 ZL 201910626036.3.授权时间：2020.9.22（国家发明专利） 9. 李莹莹，王庆，曾伟伟，王英英，石存斌，尹纪元，任燕。抗锦鲤疱疹病毒的单克隆抗体及其细胞株和应用。专利号：ZL201610033 741. 9，授权时间：2020.03.31（国家发明专利） 10. 王英英，曾伟伟，王庆，任燕，李莹莹，石存斌，朱新平。罗非鱼脑细胞系及其应用。 专利号：ZL 201810451080.0授权时间：2019.11.26（国家发明专利） 11. 王英英，王庆，曾伟伟，李莹莹，石存斌，吴淑勤。一种锦鲤脑细胞系的构建方法及其应用。专利号：ZL201510531300.7，授权时间：2019.07.16（国家发明专利） 12. 李莹莹，王庆，曾伟伟，王英英，刘春，郑树城，石存斌，宋新建，黄琦雯。一种抗锦鲤免疫球蛋白IgM的单克隆抗体及其应用。专利号：ZL201710943373.6，授权时间：2018.12.18（国家发明专利） 13. 曾伟伟，王庆，吴淑勤，王英英， 梁红茹，石存斌，李莹莹，刘春。草鱼呼肠孤病毒II型S10基因编码的重组蛋白、由其制备的多克隆抗体及应用。专利号：ZL 2013 10513653.5，授权时间：2015.5.13（国家发明专利） 14. 曾伟伟，王庆，吴淑勤，王英英，刘春，梁红茹，石存斌，李莹莹。 一种草鱼呼肠孤病毒的I型和II型的双重荧光定量PCR检测方法及其试剂盒。专利号：ZL 2013 1 0430466.0授权时间：2015.6.24（国家发明专利） |
