基本信息
姓名 |
李宁求 |
出生年月 |
1978.12 |
性别 |
男 |
硕/博导 |
硕导 |
职称 |
研究员 |
研究方向 |
水产病害防治 |
所属学院 |
动物科技学院 |
招生专业（领域） |
兽医专硕、渔业发展 |
联系方式 |
电子邮箱：liningq@126.com |
个人简介 教育、进修经历
2011.9-2015.6 上海海洋大学 水产养殖 博士
2001.9-2004.6 上海水产大学 水产养殖 硕士
1997.9-2001.6 湖南农业大学 水产养殖 学士
代表性科研项目
项目类别 |
级别(国家级、省部级) |
项目名称 |
批准部门 |
起止 时间 |
经费(万元) |
国家自然科学基金面上项目 |
国家级 |
ISKNV通过PI3K-Akt-mTOR通路调控鳜脑细胞自噬机制研究 |
国家自然科学基金委 |
2019.1-2022.12 |
59 |
国家自然科学基金青年基金 |
国家级 |
鳜源嗜水气单胞菌菌蜕投递传染性脾肾坏死病毒抗原研究 |
国家自然科学基金委 |
2013.1-2015.12 |
24 |
国家重点研发计划 |
国家级 |
水产动物重要病原及其生物学特征与毒力基础 |
国家科学与技术部 |
2018-12-2022.12 |
66.45 |
国家重点研发计划 |
国家级 |
淡水养殖鱼类重大疫病区域化免疫防控技术及示范 |
国家科学与技术部 |
2019-12-2022.12 |
59 |
广东省渔港建设和渔业产业发展专项（科技攻关与研发项目） |
省部级 |
鳜虹彩病毒、弹状病毒二联灭活疫苗创制与规模化制备关键技术 |
广东省海洋与渔业厅 |
2017.8-2017. |
100 |
广东省海洋渔业科技推广专项 |
省部级 |
淡水养殖细菌性烂鳃病疫苗研制与示范研究 |
广东省海洋与渔业厅 |
2013.7-2015.6 |
50 |
广东省农业地方标准制修订项目 |
省部级 |
鳜免疫操作技术规范 |
广东省海洋与渔业厅 |
2018.06-2019.06 |
10 |
广州市珠江科技新星项目 |
市级 |
基于全基因组策略的鱼类黄杆菌毒力相关功能基因研究及减毒活疫苗构建 |
广州市科信局 |
2012.7-2015.6 |
30 |
院基本科研业务费 |
院级 |
细胞自噬在ISKNV和SCRV复制感染中作用研究 |
中国水产科学研究院 |
2015.1-2017.12 |
23 |
代表性论文、著作等
Xiaozhe Fu, Yue Ming, Chen Li, Yinjie Niu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. Siniperca chuatsi rhabdovirus (SCRV) induces autophagy via PI3K/AktmTOR pathway in CPB cells. Fish and Shellfish Immunology 102 (2020) 381–388.
Xiaozhe Fu, Lixi Zhang, Lihui Liu, Qiang Lin, Hongru Liang, Yinjie Niu, Zhibin Huang, Ningqiu Li*. Identification of intron in ORF003 gene and its application for inactivation test of ISKNV. Microbial Pathogenesis 138 (2020) 103822
Yinjie Niu, Xiaozhe Fu, Lihui Liu, Qiang Lin, Hongru Liang, Zhibin Huang, Ningqiu Li∗. Molecular characterization and function of EGFR during viral infection process in Mandarin fishSiniperca chuatsi. Fish and Shellfish Immunology 102 (2020) 211–217
Xixi Guo, Yang Zhou, Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Yinjie Niu, Ningqiu Li*. Transcriptomic profiles reveal that inactivated iridovirus and rhabdovirusbivalent vaccine elicits robust adaptive immune responses against lethal challenge in marbled sleepy goby. Fish and Shellfish Immunology 98 (2020) 429–437
Qiang Lin, Jie Li, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Chuni Huang,Zhibin Huang, Zhaolan Mo, Ningqiu Li*. Hemorrhagic gill disease of Chinese perch caused byAeromonas salmonicida subsp.salmonicidain China. Aquaculture 519 (2020) 734775
Qiang Lin, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Yuanya Wen,,Zhibin Huang, Ningqiu Li*. Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus .Aquaculture，2020，529： 735697
Lizi Yin , Jiehao Chen, Kaiyu Wang, Yi Geng, Weiming Lai , Xiaoli Huang , Defang Chen ,Hongrui Guo , Jing Fang, Zhengli Chen, Li Tang, Chao Huang, Ningqiu Li *, Ping Ouyang.Study the antibacterial mechanism of cinnamaldehyde against drug-resistant Aeromonas hydrophila in vitro.Microbial Pathogenesis 145 (2020) 104208
Xiaozhe Fu, Xixi Guo, Shiwei Wu, Qiang Lin, Lihui Liu, Hongru Liang, Yinjie Niu and Ningqiu Li*. Non-Targeted UHPLC-Q-TOF/MS-Based Metabolomics Reveals a Metabolic Shift from Glucose to Glutamine in CPB Cells during ISKNV Infection Cycle. Metabolites 2019, 9, 174
Qiang Lin, Yongliang Zhao, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Xi ChenZhibin Huang, Li Lin, Ningqiu Li*. Development of strand-specific real-time RT-PCR for the analysis of SCRV transcription and replication dynamics. Microbial Pathogenesis，2019，129 ：146–151
Lihui Liu, Lujun Yu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Yinjie Niu, Ningqiu Li*. First report of megalocytivirus (iridoviridae) in cultured bluegill sunfish,Lepomis macrochirus, in China. Microbial Pathogenesis，2019，135：103617
Shiwei Wu, Lujun Yu, Xiaozhe Fu, Xi Yan, Qiang Lin, Lihui Liu, Hongru Liang, Ningqiu Li*. iTRAQ-based proteomic profile analysis of ISKNV-infected CPB cells with emphasizing on glucose metabolism, apoptosis and autophagy pathways。Fish and Shellfish Immunology 2018，79 ：102–111
Xiaozhe Fu, Xianqin Hu, Ningqiu Li*, Feifei Zheng, Xingxing Dong, Qiang Lin, Jiagang Tu, Lijuan Zhao, Qiwei Qin, Zhibin Huang, Li Lin*. Glutamine and glutaminolysis are required for efficient replication of infectious spleen and kidney necrosis virus in Chinese perch brain cells. Oncotarget, 2017，8（2）：2400-2412
Chen Li, Xiaozhe Fu,Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line. Fish and Shellfish Immunology, 2017，60：25-32
Qiang Lin, Xiaozhe Fu, Lihui Liu, Hongru Liang, Huizhi Guo, Shuwen Yin, Venkatesh Kumaresan, Zhibin Huang, Ningqiu Li*. Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi. Microbial Pathogenesis, 2017,107:98-105
Xia Luo, Xiaozhe Fu, Guoli Liao, Ouqin Chang, Zhibin Huang *, Ningqiu Li*. Isolation, pathogenicity and characterization of a novel bacterial pathogenStreptococcus uberisfrom diseased mandarin fishSiniperca chuatsi，Microbial Pathogenesis,2017, 107: 380-389
Hongru Liang, Xiaozhe Fu, Lihui Liu, Qiang Lin, Huizhi Guo,Yonggang Li, JiaXu Liu, Zhibin Huang, Ningqiu Li*. Inhibition of Grass Carp Reovirus Infection by DNA Aptamers against S10 Protein.Journal of Aquatic Animal Health, 2017, 29: 89–94.
Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu, Zhibin Huang, Ningqiu Li *, Jianguo Su *. The biological features and genetic diversity of novel fish rhabdovirus isolates in China. Archives of Virology， 2017，162:2829–2834
Qiang Lin, Xiaozhe Fu, Ningqiu Li, Quanyuan Wan, Wenjie Chen,Yunmao Huang, Zhibin Huang, Jun Li, Lijuan Zhao* , Li Lin*. Co-infections of infectious spleen and kidney necrosis virus and Siniperca chuatsirhabdovirus in Chinese perch (Siniperca chuatsi) . Microbial Pathogenesis, 2017,111:422-430
Huizhi Guo, Xiaozhe Fu, Qiang Lin, Lihui Liu，Hongru Liang，Zhibin Huang, Ningqiu Li*, Jianguo Su*. Mandarin fish p53: genomic structure, alternatively spliced variant and its mRNA expression after virus challenge. Fish & Shellfish Immunology, 2017, 70：536-544
Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. The pathogenicity and biological features of Santee-Cooper Ranaviruses isolated from Chinese perch and snakehead fish. Microbial Pathogenesis, 2017，112：269–273
Xiaozhe Fu, Ningqiu Li*, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang. Display of ISKNV orf086 protein on the Surface of Aeromonas hydrophila and its immunogenicity in Chinese perch (Siniperca chuatsi). Fish & Shellfish Immunology, 2016,56：286-293
Huizhi Guo, Xiaozhe Fu, Ningqiu Li*, Qiang Lin, Lihui Liu，Shuqin Wu. Molecular characterization and expression pattern of tumor suppressor protein p53 in mandarin fish, Siniperca chuatsi following virus challenge. Fish & Shellfish Immunology,2016, 51:392-400
教学科研获奖情况
（1）广谱、高效海水鱼弧菌病重组OmpK亚单位疫苗创制与转基因生物安全评价，中国水产科学研究院科技进步三等奖，第一完成人，2015年；
（2）海水养殖鱼类哈维氏弧菌、溶藻弧菌二联疫苗研究，中国水产科学研究院科技进步奖一等奖，第三完成人，2007年；
（3）淡水鱼类细菌性败血症免疫防控技术关键及产业化应用，中国水产科学研究院科技进步一等奖，第二十完成人，2013年.
（4）鳜鱼免疫与生态防控技术示范与推广，广东省农业技术推广奖二等奖，第二完成人，2019年
